Preparation of cytosolic and nuclear fractions from cultured cells and tissues is common. Cell fractionation from cultured cells is relatively easy and straightforward. However, a clear separation of cytosolic and nuclear fractions from tissues, especially frozen tissues, is challenging. The cross-contamination from frozen tissues is always an issue because of the altered tissue cellular structure caused by the freeze and thaw cycle, improper homogenization, and the use of an inadequate extraction buffer. More information: https://inventbiotech.com/products/minute-cytosolic-and-nuclear-extraction-kit-for-frozen-fresh-tissues?_pos=1&_sid=2257e16f3&_ss=r